Museum Director and interim Executive Director and CEO of the Friends of NCMNS - Dr. Denise Young
- Deputy Museum Director — Michael Sagar
- Director of Communications & Marketing — Kerry Irish
- Director of Development — Jill Diaz
- Director of Education — Christy Flint
- Director of Exhibits & Digital Media — Javan Sutton
- Director of Guest and Volunteer Services — Albert Ervin
- Director of Living Collections — Jeff Mette
- Director of the Regional Network — Charles Yelton
- Director of Research & Collections — Dr. Bob Wiese
- Director of Veterinary Sciences & Chief Veterinarian — Dr. Dan Dombrowski